This study investigated the metabolism of dehydroepiandrosterone (DHEA) and pregnenolone (PREG) in various mouse tissues and their effects on immune response. Using deuterium-labeled substrates and gas chromatography-mass spectrometry, researchers identified 7α-hydroxy-DHEA and 5-androstene-3β,17β-diol as DHEA metabolites across all tested tissues, while PREG metabolism was less extensive, with 7α-hydroxy-PREG found in
