This randomized controlled trial investigated the effects of methylfolate, pyridoxal-5'-phosphate, and methylcobalamin supplementation on homocysteine and LDL cholesterol levels in patients with MTHFR, MS, and MTRR polymorphisms. The study found significant reductions in both homocysteine and LDL cholesterol, especially in those with MTHFR C677T polymorphisms, suggesting that targeted supplementation can