Well Referenced

Tag: in vitro study

Modulating tumor reactive stroma by extracorporeal shock waves to control prostate cancer progression

This in vitro study investigated the effects of extracorporeal shock waves (ESWs) on carcinoma-associated fibroblasts (CAFs) derived from high-risk prostate cancer patients. ESW treatment at specified energy levels reduced the expression of stromal activation markers α-smooth muscle actin (α-SMA) and type I collagen (COL1) in CAFs. Conditioned media from ESW-treated

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June 26, 2025 No Comments

Tumour Cell Derived Effects on monocyte/macrophage Polarization and Function and Modulatory Potential of Viscum Album Lipophilic Extract in Vitro

This in vitro study examined the effects of Viscum album (mistletoe) lipophilic extract on the polarization and function of monocytes/macrophages derived from tumor cells. The research found that mistletoe extract significantly modulated immune cell functions, potentially enhancing the immune system’s ability to target and destroy cancer cells. While the study’s

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June 26, 2025 No Comments

Interaction of Standardized Mistletoe (Viscum Album) Extracts With Chemotherapeutic Drugs Regarding Cytostatic and Cytotoxic Effects in Vitro

This 2014 in vitro study (BMC Complementary and Alternative Medicine, PMID: 24397864) investigated the interaction between standardized mistletoe extracts (Viscum album, VAE-M and VAE-Qu) and common chemotherapeutic drugs (including doxorubicin, cisplatin, gemcitabine, docetaxel, and mitoxantrone) across multiple human cancer cell lines (breast, pancreatic, prostate, and lung). The results showed that

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June 26, 2025 No Comments

Evaluation of Chlorella as a Decorporation Agent to Enhance the Elimination of Radioactive Strontium from Body

This study explored the potential of Chlorella, a green microalga, as a decorporation agent for radioactive strontium (^90Sr). In vitro experiments showed that Chlorella effectively adsorbed ^85Sr, and in vivo studies in mice demonstrated that Chlorella significantly reduced bone accumulation of ^85Sr, promoting its excretion. These results suggest that Chlorella

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June 26, 2025 No Comments

Multi-target neuroprotective effects of herbal medicines for Alzheimer’s disease

This in vitro study examined the neuroprotective effects of seven traditional medicinal plants—Scutellaria baicalensis, Ginkgo biloba, Hypericum perforatum, Curcuma longa, Lavandula angustifolia, Trigonella foenum-graecum, and Rosmarinus officinalis—in the context of Alzheimer’s disease. Researchers evaluated antioxidant activity, enzyme inhibition related to neurodegeneration (notably GSK-3β), cytotoxicity in brain endothelial and motor neuron

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June 26, 2025 No Comments

Natural Compound Resveratrol Attenuates TNF-Alpha-Induced Vascular Dysfunction in Mice and Human Endothelial Cells: The Involvement of the NF-κB Signaling Pathway

This study explored how resveratrol, a natural polyphenol, protects against TNF-α-induced vascular inflammation in human endothelial cells and mice. At physiologically relevant concentrations (as low as 1 μM), resveratrol significantly reduced monocyte adhesion, lowered expression of key inflammatory markers like MCP-1/CCL2 and ICAM-1, and inhibited NF-κB pathway activation. In mice,

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June 26, 2025 No Comments

Ocimum tenuiflorum extract (HOLIXERTM): Possible effects on hypothalamic-pituitary-adrenal (HPA) axis in modulating stress

This study evaluated the anti-stress effects of Holixer™, a standardized extract of Ocimum tenuiflorum (Holy Basil), using in vivo models (swim endurance in mice and forced swim tests in rats) and in vitro HPA-axis assays. Results showed that Holixer™ significantly improved physical endurance, reduced stress-induced immobility, and normalized corticosterone levels.

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June 26, 2025 No Comments

Quercetin inhibits Mrgprx2-induced pseudo-allergic reaction via PLCγ-IP3R related Ca2+ fluctuations

This study explored quercetin’s effects on pseudo-allergic reactions, particularly those mediated by the Mrgprx2 receptor in mast cells. In vivo, quercetin reduced Evans blue leakage and paw swelling, indicating suppressed allergic responses. In vitro, it inhibited calcium influx and mast cell degranulation by disrupting the PLCγ-IP3R signaling pathway. Molecular docking

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June 26, 2025 No Comments

Quercetin inhibits transcriptional up-regulation of histamine H1 receptor via suppressing protein kinase C-δ/extracellular signal-regulated kinase/poly(ADP-ribose) polymerase-1 signaling pathway in HeLa cells

This study investigated quercetin’s ability to modulate histamine H1 receptor (H1R) gene expression in HeLa cells and its effects on allergy-like symptoms in TDI-sensitized rats. Quercetin suppressed histamine- and PMA-induced up-regulation of H1R by inhibiting PKC-δ activation and its translocation, reducing allergy-like symptoms in rats. In vivo, it also decreased

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June 26, 2025 No Comments

Regulation of intracellular glucose and polyol pathway by thiamine and benfotiamine in vascular cells cultured in high glucose

This study investigated the effects of thiamine and benfotiamine on glucose metabolism in vascular cells cultured in high glucose conditions. Both thiamine and benfotiamine significantly reduced aldose reductase activity, sorbitol accumulation, and intracellular glucose levels while increasing transketolase activity, thereby promoting the pentose phosphate pathway. These findings suggest that thiamine

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June 26, 2025 No Comments