This in vitro study evaluated the cytotoxic effects of (1,3)(1,4)-β-D-glucan extracted from Avena sativa (oat) on human melanoma HTB-140 cells. The compound exhibited dose-dependent cytotoxicity, with an LD50 of 194.6 ± 9.8 μg/mL after 24 hours. Apoptosis was confirmed by increased caspase-3/-7 activity and externalization of phosphatidylserine. A decrease in
