Glutamine and arginine improve permeability and tight junction protein expression in methotrexate-treated Caco-2 cells

This in vitro study explored the effects of glutamine and arginine on intestinal barrier function in Caco-2 cells treated with methotrexate (MTX), a chemotherapy agent that induces intestinal damage. Glutamine, arginine, and a few other amino acids were shown to restore the intestinal barrier integrity, as evidenced by improved transepithelial